Xpress-seq full-length scRNA-seq
Discover the benefits of our proprietary Xpress-seq technology.
Coming soon: MGI full-length scRNA-seq
Discover the benefits of our proprietary Xpress-seq technology.
Coming soon: MGI full-length scRNA-seq
World leading capture of the RNA present in cells important for detecting transcription factors in single-cell atlases and developmental biology.
Xpress-seq generates reads covering the whole RNA transcripts. Unlike 3’ end sequencing, we obtain reads along exons to inform on RNA isoforms, SNPs and mutations.
We have validated UMI-based counting for accurate RNA quantification.
Xpress Genomics unique custom robotics, enables fully end-to-end automated high-throughput processing of 384-well plates for full-length scRNA-seq at high scales (>12 plates per day).
Xpress Genomics has 10 years of experience in optimizing single-cell RNA-seq methods, including reductions in reagents to nanoliter reactions as low as 100 nL, components, and plastic consumables to arrive at competitive pricing for our customers.
For all Xpress-seq projects, we provide you with Xpress-seq cell capture plates. You only need to do cell isolation and return the plates to us; we will process your plates, sequence and process the data through our in-house pipeline.
At Xpress Genomics, we exclusively offer service for our plate-based full-length scRNA-seq technology Xpress-seq. Our founders have spent many years developing Smart-seq protocols, and developed Xpress-seq based on this experience.
For Xpress-seq service, we only work in 384-well plates that we already provide with lysis buffer to customers. Sorting of single cells of interest is performed in the customer lab and after returning the plates to us we run library prep, sequencing and data preprocessing.
In order to ensure experiment success, we also provide a detailed SOP and best practices for cell sorting.
Xpress-seq data consist of both UMI-containing reads on the 5’ end as well as internal reads covering the full gene body.
We provide you with raw reads (fastq), processed reads (error-corrected, aligned, gene-assigned), count tables and a QC report.
We offer the following sequencing options:
Xpress-seq overcomes traditional barriers and offers high-quality, full-length RNA-seq data from single-cells at scale and low cost.
Development of single-cell RNA-seq methods has traditionally focused on increasing cell numbers profiled or improving the transcriptome information. Xpress-seq is the first method to enable high-quality data for large numbers of cells. Only Xpress-seq has optimized full-length coverage of short reads along RNA transcripts and thus captures splice junctions. Thus, our users are empowered to analyze alternative splicing at high scale.
Xpress-seq single-cell service pricing per 384-well plate is determined by the number of plates submitted for processing in our bespoke automated lab. Please contact us for your personal quote! Pricing for single-cell Xpress-seq service always includes 384-well plate with lysis buffer, library preparation, sequencing and primary data processing.
Feel free to contact us to find out how we can help with your current project. You can also submit a request for a quote.
Contact UsDocuments, publications etc.
| Customer Publications | ||
|---|---|---|
| Moura et al. | Erythroid Differentiation Enhances RNA Mis-Splicing in SF3B1-Mutant Myelodysplastic Syndromes with Ring Sideroblasts | Download |
| Papanicolaou et al. | Multi-layered dosage compensation of the avian Z chromosome |
| Public Datasets | ||
|---|---|---|
| Parse Demo Data | Coming Soon: Parse Evercode WT v3 Mini data from ~5,000 human PBMCs. | |
| ATAC-seq Demo Data | Coming Soon: ATAC-seq data from 50,000 HEK293T cells. |
| Frequently Asked Questions | ||
|---|---|---|
| Our FAQs | Coming Soon | |
| Xpress-seq Data Description | Description of data formats and results files for single-cell Xpress-seq. | Download |
| Bulk Xpress-seq Data | Description of data formats and results files for bulk Xpress-seq. | Download |
| Sample requirements for our Services | ||
|---|---|---|
| Bulk RNA-seq Specs | Sample preparation and requirements for bulk RNA-seq. | Download |
| Library Submission Specs | Sample requirements for ready-made library sequencing. | Download |
| ATAC-seq Sample Prep Guide | Coming Soon: Instructions for preparing samples for ATAC-seq service. |
| Related to Xpress-seq Service | ||
|---|---|---|
| FACS sorting SOP for Xpress-seq plates. | Ensure optimal outcomes for Xpress-seq experiments with our guide to FACS sorting. | Download |
| Xpress-seq QC Information | Our guide to interpreting Xpress-seq plate QC results. | Download |
| Xpress-seq Data Description | Description of data formats and results files. | Download |
| Xpress-seq Plate Submission Sheet | For your next Xpress-seq order. | Download |
| Xpress-seq Deliverables | Specific terms and conditions for Xpress-seq service. | Download |
| For our Services | ||
|---|---|---|
| Bulk RNA-seq Submission Sheet | For your next bulk RNA-seq order. | Download |
| Library Submission Sheet | For your next ready-made library sequencing order. | Download |
| Xpress-seq Plate Submission Sheet | For your next Xpress-seq order. | Download |
© Xpress Genomics, Stockholm, Sweden. 2025
