Protein interactomics

The Proximity Network Assay (PNA)

Understanding how proteins interact and organize on the surface of individual cells is key to advancing human health. Pixelgen’s Proximity Network Assay opens a new dimension in omics by enabling high-resolution, single-cell mapping of 155 immune-related proteins in 3D. The Proximity Network Assay provides powerful insights in e.g. hematology, immunotherapy, CAR-T, autoimmunity and infectious disease, and can be used for mechanistic or biomarker studies.

Pixelgen PNA service at Xpress Genomics.

Fixation of Cells

We provide you with an SOP that only requires standard laboratory reagents. All you have to do is to isolate and fix your cells - we take care of the rest!

Library Preparation

After submitting your fixed cell suspensions to Xpress Genomics, our experienced staff will take care of running the proximity network assay and library preparation. We are a certified Pixelgen service provider, ensuring a successful experiment.

Cost-efficient Sequencing

We always pair the Pixelgen PNA assay with our cost-efficient in-house sequencing. In this way, we help you make protein interactomics even more scalable and accessible.

Data Processing

Data is processed using the official Pixelgen nf-core pipeline - all on our secure, in-house servers ensuring data integrity.

Technical Details.

The Proximity Network Assay in detail.

The Proximity Network Assay enables researchers to analyze the dynamics of the immune cell surface protein interactome in an unprecedented manner, leading to novel insights into disease mechanisms, drug mode of action response, and biomarker discovery.

Application: cell surface protein interactomics studies
Research areas: hematology, CAR-T, immunotherapy, immuno oncology, autoimmunity, infectious disease, and immunology

We support fixed cells as input originating from fresh or cryopreserved cells in suspension. Sample type can be PBMCs, isolated immune cells, bone marrow, cell lines or cells dissociated from organoids or fresh frozen tissue.

Cell input: about 500,000 – 1 M cells in suspension
Species: human
Throughput: up to 1000 cells data output per reaction
Multiplex: 155 cell surface protein markers in parallel (all against all)

We provide you with sequencing data that includes both raw reads but also processed data using Pixelgen's open source analysis pipelines.

Resolution: nanoscale (approximately ~50 nm)
Multiplex: 155 cell surface protein markers in parallel (all against all)
Data types: protein clustering, colocalization, and abundance

The Proximity Network Assay (PNA)

Pixelgen PNA service at Xpress Genomics.

Fixation of Cells

Library Preparation

Cost-efficient Sequencing

Data Processing

Technical Details.

Application & Research areas

Sample specifications

Data output

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