Combinatorial Indexing scRNA-seq

Parse Evercode v3 Technology

Learn more about Parse Evercode

Parse Evercode v3 Technology

Parse Bioscience’s Evercode split-pool combinatorial indexing is a simple, instrument-free solution to single cell sequencing. It enables scientists to push scRNA-seq past the limitations of previous technologies to scale up the samples and cells per experiment to ultra-high throughput.

A key aspect of the Parse Biosciences Evercode technology is sample fixation, which is applicable to a wide range of animal cells/nuclei. Cells/nuclei are fixed in about 65–70 minutes, and fixed samples are stable for up to six months. This means you can work on a time course study, share samples between laboratories, or accumulate samples before submitting them to Xpress Genomics for processing!

Parse Evercode service at Xpress Genomics.

Fixation of cells

We provide you with the appropriate Parse fixation reagents for your cells or nuclei along with an SOP. All you have to do is prepare your samples and isolate your cells - we take care of the rest!

Library Preparation

After submitting your fixed cell suspensions to Xpress Genomics, our experienced staff will take care of library preparation. Our team members are trained directly by Parse Biosciences in order to ensure a successful experiment.

Cost-efficient Sequencing

We always pair the Parse Evercode high-throughput single-cell assay with our cost-efficient in-house sequencing. In this way, we help you make single-cell research even more scalable and accessible.

Data processing

Data is processed using the official Parse Biosciences pipeline - all on our secure, in-house servers ensuring data integrity.

Technical Details.

Introducing Evercode Technology

Xpress Genomics is a service provider for Parse Biosciences combinatorial indexing technology.

Scalability

We offer Parse combinatorial indexing scRNA-seq fitting any experiment scale.

Evercode WT Mini: up to 10,000 cells in 1-12 samples.
Evercode WT: up to 100,000 cells in 1-48 samples.
Evercode WT Mega: up to 1,000,000 cells in 1-96 samples.
Evercode WT Penta: up to 5,000,000 cells in 1-384 samples.

Technical Details

Parse Evercode v3 technology differs from our Xpress-seq single cell technology by using the cell or nuclei as the reaction vessel.

Fixed and permeabilized cells/nuclei are loaded into a 96 well Round 1 Plate that is preloaded with well-specific barcodes. The RNA is reverse transcribed in situ with barcoded oligo dT and random hexamer primers.
The cells/nuclei are pooled and loaded into a second 96 well plate. An adaptor with a well-specific barcode is ligated to the first barcode. This is repeated to add a third barcode.
Pooled cells/nuclei are divided across several sublibraries and lysed. A fourth sublibrary-specific barcode is added during sequencing library preparation.
After sequencing, each transcript is assigned to a single cell/nuclei based on a unique combination of the four barcodes.

Proven Quality

We provide a demonstration dataset generated entirely in-house. We have sequenced >5,000 single cells from a human PBMC sample using the Evercode WT Mini kit.

Combinatorial Indexing scRNA-seq

Parse Evercode v3 Technology

Parse Evercode service at Xpress Genomics.

Fixation of cells

Library Preparation

Cost-efficient Sequencing

Data processing

Technical Details.

Ultra-high scale scRNA-seq.

Combinatorial Indexing.

Demo data.

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